A Note About Tissue Preparation in Histology

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T pepae iving tisses f mispi examinatin, a nmbe f steps ae neessay. Fist f a, the tisse is fixed with a pesevative (sh as fmain). The speimen is then imbedded in a bk f paaffin a pasti mateia. A tting instment aed a mitme sies the hadened bk f tisse int wafe-thin setins, whih ae nw thin engh t tansmit ight f mispi se.
nftnatey, iving tisses ae ess tanspaent nde a mispe. Witht a itte hep, it wd be diffit impssibe t distingish stta detais. Afte a setin f tisse is paed n a mispe side, it is stained with diffeent hemias t the vais eements f tisse es.
ne f the mst sef f these is hematxyin and esin (H+E). This pepaatin gives diffeent s t tisses, depending pn the bihemia mees pesent in the es. F exampe, the hmatin f a e nes stains dak ppish-be with hematxyin, wheeas esin stains the ytpasm and its ganees a pinkish-ed.
F this easn, mst tisse pepaatins stained with H+E (as with mst f the sides n this Web site) have dak be nei and eddish ytpasm. We wi ften mment abt the appeaane atin f the nei, abt the abndane ak f ytpasm.
The H+E stain is why mst f the sides n this Web site appea t be ?pink and be? in . F speia ppses, we wi asinay se etain stains that bing t niqe feates, sh as the myein sheath in neves the iset tisse f the paneas.
 

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